![western blot quantification using imagej western blot quantification using imagej](https://www.licor.com/bio/blog/images/f44adee968006c31a348999e3d01ad4196352575.png)
![western blot quantification using imagej western blot quantification using imagej](https://2.bp.blogspot.com/-FGV-0wfSgME/Vx5wC_e8ByI/AAAAAAAAAkI/n4ZkqQ6aoD4_m0hEVHo98DDA6WWtfSGEwCLcB/s1600/No%2BNoise%2BCorrection.jpg)
If there are more than one set of bands that you want to analyze, make as many copies of the file as there are bands that you want to analyze.Select at least 6 gridlines to help you judge whether or not the bands are horizontal.
![western blot quantification using imagej western blot quantification using imagej](https://i.ytimg.com/vi/-Zchea9xGT0/maxresdefault.jpg)
Select the angle you want (it will rotate clockwise).Rotate the image so the bands are lined up horizontally (Image > Tranform > Rotate).If you have black background and white bands, invert the image (Edit > Invert).Convert the image to 8-bit (Image > Type > 8-bit).Save the files as TIFF file using a different name than the original.